In short, I have 2 samples. The first is a negative control, so technically its value is zero (a gene that should not be expressed). The second is essentially a positive control, and thus should be expressed.
qPCR results - Ct(SMD)
- NC: n/a, 38
- PC: 35, 34
a. interpret the NC as 0 or n/a or ignore n/a and use the available data?
b. divide PC to NC in order to get relative amount?
Hope this explanation is clear..
Edited by jjj02027, 23 October 2011 - 09:23 PM.