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protein biotinylation

Started by hannna, Oct 23 2011 02:48 PM

biotinylation protein in vitro

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#1 hannna

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Posted 23 October 2011 - 02:48 PM

Hi!
Does anyone have any experience with biotinylating proteins in vitro with Bir A ligase?

I've done it already in crude extracts, but now I have to biotinylate already purified protein. Is it the same? Anyone done it before? Any tips?

Many thanks,
ciao,

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