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h9c2 cells (from atcc) problems in cell proliferation


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#1 materials researcher

materials researcher


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Posted 23 October 2011 - 04:47 AM

hi all,
is there anyone with experience in culutring H9c2 cells (CRL 1446). I got a vial of these cells from atcc and it didnt attach on the flask - found all dead. I thought it could be due to shipping problems, but yet again i purchased another fresh vial of these cells and found them still not getting attached or growing.
May i please ask for some expert advice on these cells growth behaviour?
(FYI: i have handled all types of cells including MSCs, osteoblasts, nerve cells, schwann cells, cardiomycytes etc with no problems and i am troubled by this cell line). Please suggest. thank you so much.

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