So I recently ligated my gene into a Pet32a vector using restriction sites using EcoR1 and Xho1. From another Pet vector I have to create primers for the GFP insert containing BgII and EcoR1 sites. Just wondering, once I amplify my GFP (using BgII/EcoR1 sites), how do I ligate it into my original construct..Do I cut my original constuct with EcoR1 and BgII (because looking at the vector map, Pet32a has a BgII site and ligate?)
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Cloning of GFP
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