Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Cloning of GFP

  • Please log in to reply
No replies to this topic

#1 biology_06er



  • Active Members
  • PipPipPipPipPip
  • 57 posts

Posted 23 October 2011 - 02:42 AM

Hi there,

So I recently ligated my gene into a Pet32a vector using restriction sites using EcoR1 and Xho1. From another Pet vector I have to create primers for the GFP insert containing BgII and EcoR1 sites. Just wondering, once I amplify my GFP (using BgII/EcoR1 sites), how do I ligate it into my original construct..Do I cut my original constuct with EcoR1 and BgII (because looking at the vector map, Pet32a has a BgII site and ligate?)


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.