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why is real time pcr curve like this?


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3 replies to this topic

#1 microbious

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Posted 21 October 2011 - 01:58 PM

hi

I have a problem with my results of real time pcr.
I use sybr green 1 and rotor-gene (Corbett) for relative real time pcr.
Do you have any suggestion for soloution of my problem?

please look at the picture in attach

my appreciation

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#2 jpopesku

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Posted 23 October 2011 - 10:24 AM

It seems as if the scale on your y-axis is fixed at 0 - 100. What happens if you change the scale to either "automatic" or 0 - 15?

#3 tea-test

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Posted 23 October 2011 - 11:44 PM

I would say it looks ok, but this is the linear view. try to change to log-view to set the baseline and treshold. To see the curves nicer in the linear plot look at the post of jpopesku.
tea-test: The artist formerly known as Ned Land

#4 microbious

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Posted 27 October 2011 - 10:46 PM

thanks guys

do you think i had amplification in this curve?

WHAT DO YOU THINK ABOUT RN?

tea-test please tell me more about curve nicer

and jpopesku thanks for your sugession

I wait for your answer




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