3 replies to this topic

[microbious]

hi

I have a problem with my results of real time pcr.
I use sybr green 1 and rotor-gene (Corbett) for relative real time pcr.
Do you have any suggestion for soloution of my problem?

please look at the picture in attach

my appreciation

Attached Thumbnails

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[jpopesku]

It seems as if the scale on your y-axis is fixed at 0 - 100.  What happens if you change the scale to either "automatic" or 0 - 15?

[tea-test]

I would say it looks ok, but this is the linear view. try to change to log-view to set the baseline and treshold. To see the curves nicer in the linear plot look at the post of jpopesku.

[microbious]

thanks guys

do you think i had amplification in this curve?

WHAT DO YOU THINK ABOUT RN?

tea-test please tell me more about curve nicer

and jpopesku thanks for your sugession

I wait for your answer