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[joy123]

Hi, I am a beginner of gene study, and hope to get some suggestions of the study design.

I want to test the function of a group of proteins on a disease (to compare if they are the same between healthy people and patients). The proteins are coded by 5 genes, each gene has 2-4 allels.

I want to test:
1. the prevalence of the different allels of the 5 gene loci in healthy people (~50) and patients (~50)
2. mutation of these genes in patients

I wonder what methods should I use for the two purposes? Is there a standard way? And as there are 5 genes (each gene has several exons), is there a quick method?

Look forward to your suggestions. Thanks very much!