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Gene Cloning

selection of DNA

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#1 JellowK

JellowK

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Posted 21 October 2011 - 03:57 AM

Hey again!

Having a little problem with a question i got from my
teacher.

There is three types of DNA molecules that could be ligased
during the use of a plasmid vector and DNA fragment that you want to
ligase into the plasmid

I count these

Circulair DNA fragment - consist of the fragments that you wanted to place into the vector but have
combined to a circulair structure.

Re-ligated vector - a plasmid that doesent contain a inserted fragment

Recombinant vector - a plasmid that do contain a inserted fragment

my question is how can you select these?




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