I want to examine the insulin signaling pathway for any changes in the levels of phosphoproteins in response to my treatment. I plan on using multiplex/Westerns. However, the tissue samples that I have are from mice that were not stimulated by insulin or any other growth factor. in addition, these mice were fasted overnight before killing so presumably any physiological response is muted as well. Does anyone out there have any experience with detecting phosphoproteins in unstimulated samples?
Any input is appreciated.
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phosphoprotein detection in unstimulated samples
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