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Gelatin 45% dilution

gelatin blocker

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#1 julnobody

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Posted 19 October 2011 - 05:14 AM

Hi all,


I am willing to use fish gelatin as a blocker (starting from a 45% syrupy, honey-like sticky solution, non pippetable).
Of course I need to dilute it, my question is simple, if anyone has ever used this blocker(from Sigma) how do you handle and prepare the final blocking solution?

Thank you.

#2 almost a doctor

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Posted 19 October 2011 - 05:36 AM

I've never used that particular reagent, but if it is too dense to pippet, you can just weight it. Assuming 45% is w/v it's a straight forward calculation for your dilution. If however it is a v/v concentration, you'll need to know the density of the gelatin to calculate how many grams you need of your 45% stock into your final X% dilution.

Hope this helps.

#3 bob1

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Posted 20 October 2011 - 02:53 PM

If it is "cold water fish skin gelatin", you can heat it up a bit which will make it much more liquid. I would recommend that you aliquot out a smaller amount to avoid repeat heating the whole volume.





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