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Effect of Sodium Azide and DTT in a buffer.

Sodium Azide DTT

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#1 Saurabh Raj

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Posted 19 October 2011 - 05:00 AM

Hi,

Can anyone please tell me the role of Sodium Azide and DTT in a buffer solution. I am asking it in relevance with biotin-streptavidin and dig-antidig construct formation in DNA.

Thanks
Saurabh

#2 phage434

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Posted 19 October 2011 - 05:08 AM

The sodium azide acts as an antimicrobial agent to eliminate growth of bacteria. The DTT is a reducing agent, reducing unwanted oxidation of your reactants.

#3 Saurabh Raj

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Posted 24 October 2011 - 06:44 AM

Thanks a lot :-)





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