Determining optimal digestion time for partial digestion
Posted 18 October 2011 - 11:30 AM
Posted 29 October 2017 - 01:46 AM
Procedure to obtain homogenate of T. cruzi from epimastigote culture useful for coating wells MTP: taken 1 ml of epimastigote pure culture (forms cultured at 28 ºC in LIT liquid medium), and centrifuged in 5 ml Eppendorff tube at 4000 rpm at 4 ºC for 10 min. Discard the supernatant and 1 ml of phosphate buffer saline (PBS) at pH7.2 is added, and the tube vortexed. This washing operation is repeated for three times. Then, 1 ml of PBS was added and the tube vortexed. Aliquot and store at -20 °C.