I am trying to determine the optimal digestion time for a partial digestion of T. cruzi genome with Sau3A. I don't have the gel picture but there was a time gradient, meaning different digestion times. There were two ladders, we weren't told what they were but I think one was a cDNA ladder and the other a genomic ladder for T. cruzi. Can anyone help?
Determining optimal digestion time for partial digestion
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