Hi
I am purifying a protein by SP-sepharose cation exchange column. The buffer i use to elute the protein contains potassium chloride in it. Therefore as soon as i add 4X SDS dye to my sample, it precipitates and on heating it becomes sticky and gelatinous. I need to run the protein on the gel to check where my protein is eluted. I cannot load because its too sticky and it wont run on the gel. Any suggestions?
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problem with loading the protein on to SDS-Gel
Started by
deep1985
, Oct 18 2011 10:47 AM
1 reply to this topic
#2
K.B.
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Posted 18 October 2011 - 11:07 AM
1. Can't you use sodium chloride or lithium chloride for elution?
2. You could try buffer exchange with dialysis, ultrafiltration or gel filtration (eg. PD-10 columns).
2. You could try buffer exchange with dialysis, ultrafiltration or gel filtration (eg. PD-10 columns).
