I am purifying a protein by SP-sepharose cation exchange column. The buffer i use to elute the protein contains potassium chloride in it. Therefore as soon as i add 4X SDS dye to my sample, it precipitates and on heating it becomes sticky and gelatinous. I need to run the protein on the gel to check where my protein is eluted. I cannot load because its too sticky and it wont run on the gel. Any suggestions?
problem with loading the protein on to SDS-Gel
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