I am growing a cancer cell line; they are suspension cells. I left it in the incubator over the weekend and when I came back today, I realized that the water pan in the incubator had dried.
The cells look OK under the microscope, they are healthy and growing. I am assuming that the pan probably dried on Saturday or Sunday since there was some water on Friday. Do you think my cells will be OK? Should I discard them?
0
The water pan got dry in the TC incubator. Will my cells be OK?
Started by distalless, Oct 17 2011 09:33 AM
water humidity incubator tissue culture
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 4,342 posts
Thelymitra pulchella
222
Excellent
Excellent
Posted 17 October 2011 - 02:41 PM
They should be fine. The water decreases the rate of evaporation in the flasks/plates, so that you aren't as likely to get osmotic stress on the cells.
Also tagged with one or more of these keywords: water, humidity, incubator, tissue culture
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
role of water in PCRStarted by Guest_Shrubal_* , 06 Dec 2012  PCR, water
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Which hydrogen peroxide?Started by Guest_assembler01_* , 19 Nov 2012 hydrogen peroxide, tissue culture
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Compound to Differentiate MonocytesStarted by Guest_MycoZ_* , 04 Oct 2012 Monocyte, Macrophage, phagocyte and 2 more...
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Morphology of Monocyte Derived MacrophagesStarted by Guest_MycoZ_* , 04 Oct 2012 monocyte, macrophage and 3 more...
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
|
|
|
