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The water pan got dry in the TC incubator. Will my cells be OK?

water humidity incubator tissue culture

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#1 distalless



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Posted 17 October 2011 - 09:33 AM

I am growing a cancer cell line; they are suspension cells. I left it in the incubator over the weekend and when I came back today, I realized that the water pan in the incubator had dried.

The cells look OK under the microscope, they are healthy and growing. I am assuming that the pan probably dried on Saturday or Sunday since there was some water on Friday. Do you think my cells will be OK? Should I discard them?

#2 bob1


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Posted 17 October 2011 - 02:41 PM

They should be fine. The water decreases the rate of evaporation in the flasks/plates, so that you aren't as likely to get osmotic stress on the cells.

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