I am trying to show that a compound induces translocation of glut4 in primary porcine muscle cells. I am working on blebbing giant membrane vesicles which I would like to immunostain for glut4. However, I cannot permeabilize the vesicles, and therefore need an antibody (commercial) that binds to an extracellular epitope on glut4 (if such an ab exists), and will react with pig glut4.
Does anybody know of any ab that might work for this?
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anti-GLUT4 antibody, extracellular epitope?
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