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Using conjugated Ab as primary Ab

immunofluorescence

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3 replies to this topic

#1 mago

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Posted 17 October 2011 - 03:57 AM

Hi everyone,
Do you know whether it is possible to use an alkaline phosphatase conjugated antibody as a primary antibody?
I would like to bind it a secondary, flourescently tagged, antibody.
Does the primary antibody conjugation interfere with the binding of the secondary antibody or not?
Thanks in advance,
Matan.

#2 BioMiha

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Posted 17 October 2011 - 05:44 AM

Although the conjugation process is pretty random, there are never more than a few (1-3) molecules of AP on your Ab. I have used biotinylated Abs with secondary Abs with no problem. I suppose AP-conjugates should be more or less the same.

#3 mago

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Posted 17 October 2011 - 11:44 PM

Thanks a lot.

#4 Bioss USA Antibodies

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Posted 14 November 2011 - 07:40 AM

This is definitely possible! Check here: http://www.bioss-usa...?conjugation=37





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