3 replies to this topic

[mago]

Hi everyone,
Do you know whether it is possible to use an alkaline phosphatase conjugated antibody as a primary antibody?
I would like to bind it a secondary, flourescently tagged, antibody.
Does the primary antibody conjugation interfere with the binding of the secondary antibody or not?
Thanks  in advance,
Matan.

[BioMiha]

Although the conjugation process is pretty random, there are never more than a few (1-3) molecules of AP on your Ab. I have used biotinylated Abs with secondary Abs with no problem. I suppose AP-conjugates should be more or less the same.

[mago]

Thanks a lot.

[Bioss USA Antibodies]

This is definitely possible! Check here: http://www.bioss-usa...?conjugation=37