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Ligation problem


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16 replies to this topic

#16 mahrak

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Posted 08 January 2012 - 07:27 AM

have you ever tried using PEG in your ligation reaction?

#17 sou

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Posted 22 January 2012 - 10:23 PM

have you ever tried using PEG in your ligation reaction?

when ever i do blunt end ligation, i use PEG. n if i am using the quick ligase from NEB, the ligase buffer already has PEG. Why do u ask? I should not be using PEG?




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