Posted 08 January 2012 - 07:27 AM
Posted 22 January 2012 - 10:23 PM
when ever i do blunt end ligation, i use PEG. n if i am using the quick ligase from NEB, the ligase buffer already has PEG. Why do u ask? I should not be using PEG?
have you ever tried using PEG in your ligation reaction?