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huge dot appears at the centre of every of my bands

huge dot artifect gel electrophoresis DNA band

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#1 gyhuang

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Posted 16 October 2011 - 11:55 PM

Help! huge dot appears at the centre of every of my bands I don't know why. The only suspect i'm thinking is contamination..but how could it be restricted to travel at only the centre of the band?YH inverted.jpg YH.jpg

#2 almost a doctor

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Posted 17 October 2011 - 02:14 AM

It looks to me like you've pierced your well with the end of the pipette tip while loading the sample creating a funny shape in your bands. Next time try no to put the tip so deep in the well.

just my 2 cents!

#3 gyhuang

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Posted 17 October 2011 - 02:28 AM

oh I see. I think that must be problem.I recalled that I wanted to make sure that the DNA I was loading does not escape too much out of the well so i purposely place the tip slightly deeper(not pierce the bottom of well though). Thankx alot:)





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