We have finished the transformation process.
It leaves us with three choices:
the diluted positive control plasmid DNA with the concentrated one, as well as the DNA we wish to analyze.
So which one should I choose to calculate the transformation efficiency?
I think it is the diluted plasmid DNA but I don't know why.
No replies to this topic
Also tagged with one or more of these keywords: PCR
![]() |
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
High fidelity PCR trouble shootingStarted by Guest_uday_* , Yesterday, 06:59 PM |
|
|
|
![]() |
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Reproducible Non-Specific PCR ProductStarted by Guest_Epigeneticist_* , 20 May 2013 |
|
|
|
![]() |
Protocols and Techniques Forums →
Tissue and Cell Culture →
Housekeeper for HEK cells?Started by Guest_Stephp_* , 17 May 2013 |
|
|
|
![]() |
Protocols and Techniques Forums →
Molecular Cloning →
Cloning large fragmentsStarted by Guest_bknm_* , 15 May 2013 |
|
|
|
![]() |
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Can't get PCR with large overhang primers to workStarted by Guest_EtOH_* , 10 May 2013 |
|
|














