Hi guys!,
I was wondering if you could help me to figure out what is wrong. I ran a gel on Friday afternoon for 40 minutes- 100V, stained it using ethidium bromide for 10 minutes and when I check the gel it was EMPTY:( I couldn't even see the ladder. The gel was 1%. What do you think happened??
Help!
Thanks!
0
1 reply to this topic
#2
phage434
-
- Global Moderators
-
- 1,805 posts
Veteran
130
Excellent
Excellent
Posted 16 October 2011 - 11:20 PM
Ran the gel backwards? Used water to make the gel instead of buffer? Missing loading buffer so you DNA floated out of the wells?
Also tagged with one or more of these keywords: BrEt, PCR gel
Protocols and Techniques Forums →
Cell Biology →
Does this qualify as a whole cell-based assay?Started by Guest_tdcmmon_* , 24 Apr 2012  bioluminescence, BRET, whole cell and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Cells in suspension vs. platedStarted by Guest_tdcmmon_* , 16 Apr 2012 HEK293, BRET, plating and 1 more...
|
|
|
