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Empty agarose gel after electrophoresis

BrEt PCR gel

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#1 Ladymarge

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Posted 16 October 2011 - 08:00 PM

Hi guys!,

I was wondering if you could help me to figure out what is wrong. I ran a gel on Friday afternoon for 40 minutes- 100V, stained it using ethidium bromide for 10 minutes and when I check the gel it was EMPTY:( I couldn't even see the ladder. The gel was 1%. What do you think happened??

Help!

Thanks!

#2 phage434

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Posted 16 October 2011 - 11:20 PM

Ran the gel backwards? Used water to make the gel instead of buffer? Missing loading buffer so you DNA floated out of the wells?





Also tagged with one or more of these keywords: BrEt, PCR gel

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