Does anyone have a good protocol for cell fractionation so you can get cytoplasmic, nuclear and chromatin fractions?
2 replies to this topic
#1
Posted 14 October 2011 - 07:58 AM
#2
Posted 15 October 2011 - 06:06 AM
http://www.uni-kl.de...teomcs_2005.pdf
Isabelle Guillemin et al., 2005
(A subcellular prefractionation protocol for minute amounts of mammalian cell cultures and tissue)
Isabelle Guillemin et al., 2005
(A subcellular prefractionation protocol for minute amounts of mammalian cell cultures and tissue)
#3
Posted 17 October 2011 - 07:47 AM
Thanks, still need a protocol for chromatin isolation from nuclei













