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Cell fractionation

nuclear chromatin

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#1 RM82

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Posted 14 October 2011 - 07:58 AM

Does anyone have a good protocol for cell fractionation so you can get cytoplasmic, nuclear and chromatin fractions?

#2 Kamran

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Posted 15 October 2011 - 06:06 AM

http://www.uni-kl.de...teomcs_2005.pdf


Isabelle Guillemin et al., 2005
(A subcellular prefractionation protocol for minute amounts of mammalian cell cultures and tissue)

#3 RM82

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Posted 17 October 2011 - 07:47 AM

Thanks, still need a protocol for chromatin isolation from nuclei





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