Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

- - - - -

RNA isolation from PBMCs


  • Please log in to reply
2 replies to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
0
Neutral

Posted 28 July 2001 - 09:00 PM

Does anyone have a good protocol for isolating total RNA from isolated PBMCs?

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
0
Neutral

Posted 31 July 2001 - 09:00 PM

I have been using the Sigma RNA purification kit, but not for PBMCs. Might be worth a try though.

#3 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
0
Neutral

Posted 20 August 2001 - 09:00 PM

use the standard TRIzol protocol (life technologies).  It worked for me.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.