I wanted to transfect the HEk cells with lentiviral vector.
Before addition of the transfection complex I forgot to treat the cells with chloroquine.
what is the purpose of chloroquine in transfection? Can anyone please tell me whether the transfection has taken place, as i forgot to add chloroquine?
Thanks in advance.
Edited by Myco Rax, 13 October 2011 - 11:14 PM.