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Quantifying DNA from Buccal Swabs

DNA quantification buccal swabs

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#1 ca.dreaming

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Posted 13 October 2011 - 04:57 PM

So I'm collecting DNA from Buccal Swabs but having some trouble quantifying it. Is there any DNA extraction kit that allows the use of Nanodrop???
Otherwise, Im trying to create a standard curve for fluorescence using Hoechst Dye. Right now I've got DNA concentrations between 0 and 2.6 micrograms/ml and 1 mg/ml Hoechst Dye in a 100microliter well with buffer.
352nm excitation, 461nm emission wavelengths...

But my values are all over the board and not linear at all.
Any idea where I could be going wrong?

Any help would be much appreciated!
Best,
Constance

#2 phage434

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Posted 14 October 2011 - 06:44 AM

I'd make sure my quantitation was working by testing serial dilutions of a known DNA concentration. Those concentrations sound very low, and are likely near the limit of detection, but a serial dilution test will tell you if that is true.





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