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Endoplasmic Reticulum Isolation Protein Concentration

ER endoplasmic reticulum isolation

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#1 wallman

wallman

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Posted 13 October 2011 - 02:20 PM

Hi everyone. I've been doing ER preps with Jurkat cells using the Sigma-Aldrich Endoplasmic Reticulum Isolation kit. I've been getting pretty consistent protein concentrations of about .4 ug/uL, but a friend of mine who works with Hela cells says that she usually gets about 1 ug/uL. Are my results normal for Jurkat cells, or should I also be getting 1 ug/ul?

Thanks.

EDIT: I've done it a few times so far, with either 100 or 200 million cells. I get about the same protein concentrations with 100 or 200, except that I get more volume with 200.

Edited by wallman, 13 October 2011 - 02:22 PM.






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