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Extrct RNA from fungus in broth medium

RNA extraction

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#1 afRNA

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Posted 12 October 2011 - 07:07 PM

Hi friends,

I'm trying to extract the RNA from a fungus. But, I culture the fungus in broth medium (liquid culture). so, I need to know that, the protocol for isolation and purification of total RNA from broth...
I use the avicel broth
Fungus is T.asperellum
Please give suggestions..
pls help me...

Regardz
Afaq

#2 Kamran

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Posted 12 October 2011 - 11:42 PM

Please follow this link;

http://www.springerl...x2/fulltext.pdf

Isolation of Fungal Nucleic Acids
by Surapareddy Sreenivasaprasad


From: The Nucleic Acid Protocols Handbook
Edited by: R. Rapley © Humana Press Inc., Totowa, NJ

#3 afRNA

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Posted 13 October 2011 - 03:23 AM

thank you very much and pls give me the link for download The Nucleic Acid Protocols Handbook on internet..

Edited by afaq.mohamed@yahoo.com, 13 October 2011 - 03:30 AM.






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