Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Help needed- EBs were too small for differentiation

stem cells EB formation

  • Please log in to reply
3 replies to this topic

#1 minerva_29

minerva_29

    member

  • Active Members
  • Pip
  • 20 posts
1
Neutral

Posted 12 October 2011 - 05:09 AM

Hi!

My task was to form EB from mouse ipsc (2A-EGFPTg-3F) and from there, someone will use the EBs to differentiate to hematopoeitic cells. But, when we checked it, EBs were formed but they were too small, too small that they couldnt be used for differentiation.

What could have been the cause for this? and how can I improve it?

Thanks a lot!

#2 Kamran

Kamran

    Kamran

  • Active Members
  • PipPipPipPipPip
  • 41 posts
2
Neutral

Posted 12 October 2011 - 10:11 PM

Dear,
Feed them regularly.
Which tissue culture plates are you using for EBs formation?

#3 minerva_29

minerva_29

    member

  • Active Members
  • Pip
  • 20 posts
1
Neutral

Posted 12 October 2011 - 10:54 PM

Thanks!

I used the round bottom low cell bindin 96 well plate. i seeded 1000 cells in 1row and 3000 cells in the 2nd row. after 2 days, i added 80uL of EB medium. 3 days later i changed the medium.

#4 enock02

enock02

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 19 March 2012 - 06:47 AM

Try to seed more cells and use larger plates, the cells will clump together as time goes.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.