Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

nitroblue tetrazolium assay

NBT Macrophage THP

  • Please log in to reply
1 reply to this topic

#1 swizla21

swizla21

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 11 October 2011 - 03:32 PM

Has anyone undertaken nitroblue tetrazolium assay ?

If so any tips would be greatly appreciated.

#2 Zagami Francesco

Zagami Francesco

    member

  • Active Members
  • Pip
  • 15 posts
2
Neutral

Posted 11 June 2015 - 08:03 AM

UTIs-screening tests : to screen UTIs we use NBT reduction test, add 80 μl of 0.75 mM NBT, 110 μl of 20 mM Hepes buffer, pH 7.4, and 10 μl of 10% Triton X-100 to 200 μl of fresh whole urine at room temperature and incubate for three minutes. A positive NBT result is a color change to blue-violet.
 







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.