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Iipoprotein determination

lipoprotein electrophoresis TLC HPLC

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3 replies to this topic

#1 swizla21

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Posted 11 October 2011 - 03:27 PM

Hi,

Im currently trying to separate lipoproteins (HDL, VDL, LDL). Once separated is there anyway to determine what I have separated is that particular type of lipoprotein?

I am not in need to quantify the lipoprotein just prove with some degree that it is either HDL, LDL, VLDL.

I shall then dilute the lipoprotein and place them in to a boyden chambre and analyse to see if any THP-1 differentiated macrophages migrate towards them.

Riz

#2 mdfenko

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Posted 12 October 2011 - 11:38 AM

as it shows in the pictures with your procedure, the bands will be prominent and you'll be able to determine which is which by their position in the gradient.
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#3 swizla21

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Posted 12 October 2011 - 05:07 PM

But is there a catagorical way to determine what they are. For example if i took samples off the band and gave them to someone but I did not label them. Could i performa test to one confirm they are lipids and secondly that they are either LDL, HDL etc? for some reason im thing of some sort of electrophoresis procedure!

#4 mdfenko

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Posted 13 October 2011 - 05:09 AM

the papers referenced (and linked) in one of your other threads show electrophoretic identification of the three lipoproteins.

Edited by mdfenko, 13 October 2011 - 05:15 AM.

talent does what it can
genius does what it must
i do what i get paid to do





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