Dropped cells into liquid nitrogen tank
Posted 11 October 2011 - 09:53 AM
Any suggestions for ways in which I can retrieve these vials from the bottom of the tank of liquid nitrogen?
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Posted 11 October 2011 - 10:20 AM
Posted 12 October 2011 - 08:08 AM
Take the dewar outside in the open air i.e. a deliveries yard for example
Use full face protection and long gloves.
Get a spare dewar, fill it with LN2 and place the columns from the original dewar in to that one.
Pour off VERY SLOWLY as much of the LN2 as you can from the dewar that has the cryovials at the bottom.
Once empty up-end the dewar and the vials will fall out.
Quickly reposition them into the columns/canes.
Refill with LN2
YOU MUST HAVE AN EMPTY SPARE DEWAR AND ALSO HAVE AN OUTSIDE SPACE.......WHICH IS NOT A BASEMENT AREA.
Hope this is useful.
Posted 23 December 2011 - 09:34 AM
Hope this is useful for your problem