4 replies to this topic

[Podge]

Hey guys,

I'm looking for a small intestinal cell line that forms nice and tight monolayers that I can use for transport studies.

We have Caco-2 in the lab and used them a lot, but 1. they are colorectal and 2. they don't express a small intestinal receptor I'm looking for.

Anybody worked in the field and knows another cell line matching my criteria?

Thank you!

[zienpiggie]

What kind of intestinal receptor are you looking at?

[Podge]

Hey,

sweettaste receptors and the likes

[zienpiggie]

Do you know any intestinal cell line that would express sweet taste receptors other than Caco-2?

[Podge]

Nope. That's what I want to find out after I've found a duodenal or ileal cell line that makes nice barriers for transport studies