longer running of proteins in SDS PAGE!
Posted 10 October 2011 - 02:45 PM
It seems that I lost one band, the lower one on which I should do actin W.B.
Is there possibility to perform anyway the western blot even the marker band of 42kd is lost during running! Or i need to do 2 western blot on that filter and run another gel for 3 appropriate W.B.
thanks for help
Posted 10 October 2011 - 02:57 PM
As for this time, I would say that you may well have lost the actin, so you will probably need to run another gel, in which case you could probe that one for all three.
Posted 26 October 2011 - 02:47 AM