Knock down efficiency
Posted 10 October 2011 - 01:27 PM
I try to knock down a protein by shRNA. As I do not have the antibody, so I use the real-time PCR to check the knock down efficiency. After infection, I can knock down the target gene mRNA expression level to 30% compared to Non-target control.
Do you think this will lead to decrease the protein express level?
Posted 10 October 2011 - 02:41 PM
Posted 10 October 2011 - 03:15 PM