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Knock down efficiency


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#1 zyloveszy

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Posted 10 October 2011 - 01:27 PM

Hi there,

I try to knock down a protein by shRNA. As I do not have the antibody, so I use the real-time PCR to check the knock down efficiency. After infection, I can knock down the target gene mRNA expression level to 30% compared to Non-target control.
Do you think this will lead to decrease the protein express level?

Thank you.

#2 bob1

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Posted 10 October 2011 - 02:41 PM

You can't be sure unitl you look with a western blot. If your protein is normally particularly highly expressed, you may (and it would need to be validated first) be able to see a difference on a coomassie stain of a gel, if you are lucky!

#3 zyloveszy

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Posted 10 October 2011 - 03:15 PM

Thank you. I will wait for my antibody, and the same time I plan to use these KD cells do some phenotype analysis.




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