Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Understanding osmolality

contraction osmolality potassium chloride sucrose

  • Please log in to reply
1 reply to this topic

#1 Jimbeezee



  • Members
  • Pip
  • 1 posts

Posted 10 October 2011 - 06:41 AM


I am currently trying to induce cell contraction by treating my cells with 60 mM potassium chloride (KCl) in pre-made medium. Since I don't have a way of reducing NaCl content in the medium to compensate for the increase in osmolality, I need to do a control experiment in which I treat the cells with sucrose, so that I can show contraction is due to direct effect of KCl and not the increase in osmolality. However I don't really understand how calculations of osmolality works . . . Is adding 60 mM KCl equivalent to adding 60 mOsm? How much sucrose do I need to increase osmolality by the same amount? I would really appreciate the help, thank you!

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,707 posts

Posted 10 October 2011 - 02:36 PM

KCl in solution produces 2 ionic forms, K+ and Cl-, which are independent osmoles, thus a 1M KCl solution is a 2 Osm solution...

Also tagged with one or more of these keywords: contraction, osmolality, potassium chloride, sucrose

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.