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THP-1 macrophage transient transfection

THP-1 transfection

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#1 vaas22

vaas22

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Posted 09 October 2011 - 05:33 PM

Hi,

I am currently working with THP-1 cells. I differentiate them with PMA overnight, wash off the PMA and then infect the adherent macrophages. Typically my experiments last between 2-3 days. I am now starting to look at introducing dominant negative plasmids and siRNA into these cells. During literature search, I have come across protocols that involve transfection of these cells both before and after differentiation. I am not sure which protocol is the best to use. I would appreciate any advice I can get on how to transfect these cells!!

Thanks,
vaas22





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