3 replies to this topic

[leonius]

Hi to everybody,
I will soon start chromatin immunoprecipitations and am just wondering what exactly an IgG control does tell me.
For example I am working with murine cells. So I will have to use a mouse IgG. But what exactly is this IgG antibody control? Is it a mixture of unspecific antibodies? How is it generated/produced?
I don't get the exact theory behind this. I did not find an answer in the internet.
So would be great, if someone could explain it to me.

[bob1]

It tells you that what you are precipitating is not something which just binds to IgG...

[leonius]

Ok, maybe I did not express myself very good: I understand what it tells me.
I just don't understand, what the IgG control exactly is. I mean, my antibody which I use for precipitation may als be of IgG type.
So what is this IgG control in a physiological context? Is it a mixture of IgG molecules isolated from the animal of which's cells I perform the ChIP? E.g. I buy a from the manufacturer as "normal mouse IgG" prescribed IgG control. Is this just the whole amount of IgGs isolated from mouse?

[bob1]

Basically yes, it is the normal complement of IgG that is floating around in the serum at any one time.