17 replies to this topic

[OA17]

Hello!

I have a very simple question: Does somebody know if it is possible to graft a herbaceous species (such as Nicotiana) onto a woody species (such as Prunus)? It would not need to be an ever-lasting graft, it is just to transmit a virus from Nicotiana into Prunus, because the typical method of mechanical inoculation is not working and I would like to try other approaches.

If you have some other idea, I would really like to hear it!

Thank you very much and have a nice weekend!

[hobglobin]

No idea if it works, but I know that some plant viruses are transferred by "particle bombardment", i.e. mechanically by "shooting" the DNA e.g. with high air pressure into plant material. With a google search you'll find lot's of papers and protocols.
Good luck.

[OA17]

Thanks Hobglobin, but in this case, it is an RNA virus, and even though I have built a cDNA infective clone and I have shot the trees, it seems that it is very difficult for the virus to replicate in Prunus (and very easy to replicate in Nicotiana). That's why I was thinking of grafing.

I think I will try anyway. After all, it's a cheap method and I am quite curious!

Thanks again for the reply!

[pito]

Even if the grafting works, its still possible the infection wont work.
It depends on the Plasmodesmata, if they block the enterence of the virus then you are still in trouble.µ

Are you sure that the specific virus you use is capable of infecting that tree ?

[pito]

OA17, on 07 October 2011 - 06:56 AM, said:

Hello!

I have a very simple question: Does somebody know if it is possible to graft a herbaceous species (such as Nicotiana) onto a woody species (such as Prunus)? It would not need to be an ever-lasting graft, it is just to transmit a virus from Nicotiana into Prunus, because the typical method of mechanical inoculation is not working and I would like to try other approaches.

If you have some other idea, I would really like to hear it!

Thank you very much and have a nice weekend!

I almost forgot:
http://www.tandfonli...060669609500600

Check it out.

Grafting between different families/species is hard, but it can be done.

[OA17]

Thank you very much Pito!
I will take a look, for sure!

[OA17]

Hey Pito, I have searched for this paper, but it has no free access. I can't find the e-mail of the authors to ask them for a free copy or some information. Are you able to download it? In case you can, would it be a big incovenience for you to send it to me?
Thanks again!

[pito]

OA17, on 17 November 2011 - 05:50 AM, said:

Hey Pito, I have searched for this paper, but it has no free access. I can't find the e-mail of the authors to ask them for a free copy or some information. Are you able to download it? In case you can, would it be a big incovenience for you to send it to me?
Thanks again!

Here it is:
http://hotfile.com/d.../paper.pdf.html

[OA17]

Thanks a lot!!!! I hope it works!

[toejam]

I hadn't heard of grafting between different species, but if it's published I suppose it's possible. there are many different kinds of grafting. probably one that could work is a bud grafting, but then meristematic tissue is normally virus-free. I guess you'll have to try different grafting methods. good luck!

[pito]

toejam, on 05 December 2011 - 04:41 AM, said:

I hadn't heard of grafting between different species, but if it's published I suppose it's possible. there are many different kinds of grafting. probably one that could work is a bud grafting, but then meristematic tissue is normally virus-free. I guess you'll have to try different grafting methods. good luck!

Its possible, but hard and the grafts dont survive that long.
Its more something done for research rather then more commercial things.

[toejam]

you're absolutely right pito, i hadn't thought about it before, but i knew i had seen it somewhere: in cacti

[pito]

toejam, on 05 December 2011 - 04:36 PM, said:

you're absolutely right pito, i hadn't thought about it before, but i knew i had seen it somewhere: in cacti

haha, indeed, I didnt even think about this.

But with cacti its indeed done very often they graft between species.

[hobglobin]

perhaps within a plant family (Cactaceae) it's easier than between different families? Or anyway all cacti are the same species, but they didn't realised it so far?

[toejam]

for sure they are, same as all spiders..  

species are just an anthropocentric creation after all. however, grafting does not have the same opinion..