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little or degraded dna in transformants

degraded dna transformants retroviral vector parental vector OK

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#1 taikyokoroger



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Posted 05 October 2011 - 04:51 PM

any ideas?
i am cloning a small insert (by restriction digest) into a retroviral expression vector. many colonies i pick either do not grow or contain little DNA or appear as a smear after digest on the gel. after several attempts, i transformed the parental retroviral expression vector, performed a miniprep, and ran out the digest on the gel. IT LOOKS FINE.

i am using stbl2 from invitrogen
i have successfully cloned before, as well as into this plasmid.

however i have never run into this problem before and i am at a loss as to what to try next.

thanks for any ideas

Also tagged with one or more of these keywords: degraded dna, transformants, retroviral vector, parental vector OK

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