i am cloning a small insert (by restriction digest) into a retroviral expression vector. many colonies i pick either do not grow or contain little DNA or appear as a smear after digest on the gel. after several attempts, i transformed the parental retroviral expression vector, performed a miniprep, and ran out the digest on the gel. IT LOOKS FINE.
i am using stbl2 from invitrogen
i have successfully cloned before, as well as into this plasmid.
however i have never run into this problem before and i am at a loss as to what to try next.
thanks for any ideas
Submit your paper to J Biol Methods today!
little or degraded dna in transformantsdegraded dna transformants retroviral vector parental vector OK
No replies to this topic