2 replies to this topic

[nightingale]

Dear All,

i have received a set of primers ordered from Invitrogen..
for the first time!
am used to order from IDT, & when resuspending i simply enter the website & fill the sheet with the required info. and get my solution ...
but, now ... am kind of not sure ...
does invitrogen has a similar website ?? i explored with no fruitful results ...

so :
here is the certificate of analysis ;
scale of synthesis : 50 nmol
the reverse primer's nmoles = 40.0
the forward primer's nmoles = 39.6
now, to make 100 (uM/L) or (pmol/ul) i will :-
add 400 ul nuclease free water /TE buffer to the reverse  &
396 ul to the forward, right ???

grateful ...

Edited by nightingale, 05 October 2011 - 10:58 AM.

[allynspear]

That's right. The only unit you didn't give is the easiest to use which is nmoles/mL.

   40.0 nmoles
--------------------    =    0.400 mL = 400 uL
100 nmoles/mL


Enjoy your primers.

[nightingale]

thank You