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Cell cycle arrested... what happens then ?

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#1 v_711



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Posted 04 October 2011 - 11:22 PM

Hi !

I am having a few questions about observations I made recently.
I work with HK-2 cell line (epithelial cells from proximal tubule of the kidney) which are (for the sake of my experiments) submitted to oxidative stress.
We know oxidative stress leads to DNA damages ; and these damages may in turn lead to cell cycle arrest.
What I observed (and what is already well described in many papers relative to my subject) was a cell cycle arrest at G2/M checkpoint.

This arrest supposedly occurs to let the cell try to repair its DNA.

Thus, my question is : what happens in a cell whose cell cycle is arrested at G2/M checkpoint ?

I believe background functions are still operating (Krebs cycle, ion equilibrium pumping, ...).

But what about specific functions such as specific proteins expression.
For instance, cadehrin occuring in tight junctions: are these supposed to be still present ? (as the cell was almost about to divide)
And what about cytokine excretion? Should it excrete twice the amount as G0 or G1 cell ?

Thank you in advance for your answers.


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