Posted 04 October 2011 - 11:13 PM
for Inoue method of Competent cell preparation how to add MnCl2.4H20 while making the Transformation Buffer? Is it to be added sepaartely or in any other form?
please help.thanks in advance.
Posted 05 October 2011 - 07:11 AM
Posted 05 October 2011 - 01:48 PM
You can also just filter sterilize the whole lot.