Hi,
During DNA precipitation i always incubate it in isopropanol overnight at 4oC, and instead of getting a nice pallet of DNA my DNA either be in goo form or jelly form. Is there any different between precipitate DNA for a couple hour in room temp and doing it overnight at 4oC. If there is whats is the reason.
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#2
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Hmmm, I think it's working
Posted 05 October 2011 - 01:51 PM
IPA pellets are often gel like. It doesn't really matter which temperature you ppt at, the amount of extra DNA (or RNA) you manage to precipitate is minimal.
#3
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Posted 09 October 2011 - 11:12 PM
thanks bob1 =)
What about the time period then, will it effect the yield of DNA.
What about the time period then, will it effect the yield of DNA.
#4
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Hmmm, I think it's working
Posted 10 October 2011 - 01:59 PM
In theory yes, in practice, not really - you might be able to squeeze a few extra micrograms out, but it also increases the risk of precipitating any extra proteins or RNA that are still there.
#5
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Posted 18 October 2011 - 09:42 PM
What happen to my DNA is that, after kept in -20 it tend to be gel-like and i can actually spins down the gel and collect the solution above it. When i measure the OD of the solution i can still get a good yield of DNA. AS for the gel i'm not sure whether it is DNA, protein or salt.
oh, and the purity is way below 1.8 (A260/280)
oh, and the purity is way below 1.8 (A260/280)
