I am using E coli DNA for my experiments. What I need is to restrict 24Kb E coli DNA to two equal halves and then to label one half (24Kb) DNA with biotin and DIG. Then I attach this DNA with streptavidin and anti-digoxigenin coated beads. My problem is that the protocol that I am following doesnot give me strong enough coupling between the beads and DNA and the binding breaks very quickly. Can anyone suggest where to find detailed protocol. I would appreciate any help. Help on preperation of beads will be also very much appreciated.
Thanks
Saurabh
Submit your paper to J Biol Methods today!
Protocol for labelling DNA with DIG and biotin
Started by Saurabh Raj, Oct 04 2011 07:05 AM
DIG DNA Biotin
No replies to this topic
#1
Saurabh Raj
-
- Active Members
-
- 12 posts
member
1
Neutral
Neutral
Also tagged with one or more of these keywords: DIG, DNA, Biotin
 |
Protocols and Techniques Forums →
Molecular Cloning →
Question: Can I cut out rb glob PA TerminatorStarted by Fabian Yu, 21 Jan 2015  cloning, dna, plasmid and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Low Yield MiniprepStarted by cytheon, 20 Jan 2015 DNA, miniprep, maxiprep
|
|
|
|
|
|
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
DNA SDS-PAGE electrophoresisStarted by trilobite, 16 Dec 2014 DNA, SDS-PAGE, INSERTION
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
How can we expand molecular cloning further?Started by SarahS_, 30 Nov 2014 molecular biology, cloning and 3 more...
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
All I get is supercoiled DNA...Started by Michelle F, 10 Nov 2014 cloning, supercoiled, dna and 2 more...
|
|
|
