I am using E coli DNA for my experiments. What I need is to restrict 24Kb E coli DNA to two equal halves and then to label one half (24Kb) DNA with biotin and DIG. Then I attach this DNA with streptavidin and anti-digoxigenin coated beads. My problem is that the protocol that I am following doesnot give me strong enough coupling between the beads and DNA and the binding breaks very quickly. Can anyone suggest where to find detailed protocol. I would appreciate any help. Help on preperation of beads will be also very much appreciated.
Thanks
Saurabh
Submit your paper to J Biol Methods today!
Protocol for labelling DNA with DIG and biotin
Started by Saurabh Raj, Oct 04 2011 07:05 AM
DIG DNA Biotin
No replies to this topic
#1
Saurabh Raj
-
- Active Members
-
- 12 posts
member
1
Neutral
Neutral
Also tagged with one or more of these keywords: DIG, DNA, Biotin
 |
Protocols and Techniques Forums →
Molecular Biology →
RNA pull downStarted by laura77, 23 Feb 2015  RNA, RNA pull down assay, biotin
|
|
|
|
|
|
Protocols and Techniques Forums →
Protein and Proteomics →
RNA/protein pull downStarted by laura77, 19 Feb 2015 RNA oligo, Biotin and 2 more...
|
|
|
|
|
|
Student Forums (for study, homework and non technical questions) →
-Molecular Biology- →
How can DNA purity be checked? (original title "PROTOCOLS")Started by Eagle 1, 11 Feb 2015 How can the purity of ex, DNA
|
|
|
|
|
|
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
Antibody-Biotinylation, detection via SDS-GelStarted by petertob, 02 Feb 2015 Antibody biotinylation and 4 more...
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Question: Can I cut out rb glob PA TerminatorStarted by Fabian Yu, 21 Jan 2015 cloning, dna, plasmid and 1 more...
|
|
|
