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Protocol for labelling DNA with DIG and biotin

Started by Saurabh Raj, Oct 04 2011 07:05 AM

DIG DNA Biotin

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#1 Saurabh Raj

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Posted 04 October 2011 - 07:05 AM

I am using E coli DNA for my experiments. What I need is to restrict 24Kb E coli DNA to two equal halves and then to label one half (24Kb) DNA with biotin and DIG. Then I attach this DNA with streptavidin and anti-digoxigenin coated beads. My problem is that the protocol that I am following doesnot give me strong enough coupling between the beads and DNA and the binding breaks very quickly. Can anyone suggest where to find detailed protocol. I would appreciate any help. Help on preperation of beads will be also very much appreciated.

Thanks
Saurabh

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