turbid DNA from maxiprepmaxi
Posted 02 October 2011 - 07:57 AM
i got a very weird DNA product from Maxi prep,
i used the Nucleobond Xtra EF maxi kit, and after precipitation with isopropanol and washed with 70perc etOH,
i got a very white DNA pellet, after drying it turns transparent but still slightly white.
i dissolved in nuclease water and checked the ratio is very good, 1.87 at 260/280 and 2.2 at 260/230, however,
the dna looks a bit milky and turbid, not very clear and transparent look like water ( which i usually got with qiagen kit), i was wondering what would be the possbile reasons for this, as it doesnt seems like contaminated by protein. can i transfect cells with this kind of milky plasmid? can anyone has the same experience like this, appreciate for any help
Posted 02 October 2011 - 12:01 PM
...if you centrifuge the solution there is no precipitate?
Posted 02 October 2011 - 06:40 PM
i did the spinning, and it got a very viscous pellet, the supernatant is still keeping the same concentration of the dna,
but i am still wondering what would that be so that i could avoid this issue next time. is the pellet SDS?
Posted 03 October 2011 - 08:16 AM
You can try incubating at moderate temperature 37°-50°C with slow shaking ...or do some freezze-thaw cyles ...this helps most of the time. You can check if the DNA concentration increases with time.