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[biology_06er]

Hi there,

I recently did a miniprep using a kit (Zyppy Plasmid Miniprep Kit---http://www.zymoresea.../pdf/D4036i.pdf page 5 for the protocol) and when I went to use the nanodrop to measure the concentration of DNA, I got confused as to what to use as a blank...last time I am preettyy sure I used one of the buffers from the kit but seeing as I wasn't sure this time, I asked someone who said it was ok to use water...seeing as the blank should be what you dissolve your sample in, shouldn't I use the "elution buffer"?

cheers
b_06er

[phage434]

In principle, yes.  In practice, it will make little difference.  The elution buffer is simply buffered water, typically Tris-Cl 10 mM.  It will not absorb significantly at 260 or 280 nm.