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Anyone use Qiagen pyrosequencer?

nucleotide qiagen pyrosequencer

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#1 PostDocTrauma



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Posted 28 September 2011 - 12:54 PM

Hi all,

We have a problem with the pyrosequencer. As we are looking for methylation, we are always running out of the T nucleotides and Qiagen refuse to sell us single components of the kit, we have to buy a whole new kit.

Does anyone have any solutions?

We want to test the pyrosequencer with different sequencing grade nucleotides but I'm hoping someone else will have a solution to this problem.

Many thanks!

#2 methylnick



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Posted 11 October 2011 - 02:40 AM

try also sequencing the complementary strand? i.e.: biotinylate the opposing strand and then prime off that?

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