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Colony forming Units nd Plating doubling Time (PDT)

cfu PDT

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#1 Meera

Meera

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Posted 27 September 2011 - 09:28 PM

Please help me out in finding the colony forming unit of the MSC where the initial cells seeded per plate (capacity of 2 ml) was 200 cells.

please help me out

the count after trypsinization was calculated as


Cells per ml: average count per square x dilution factor x 10^4
7/4x 5x 10^4
8.75 x 10^4

Total cells= cells per ml x original volume from which sample was removed
8.75 x 10^4 x 1ml
8.75 X10^4 total cells

CFU efficiency = counted CFU-F/cells originally seeded x 100

8.75 x 10^4/200 cells x100 = 4.375 x10^4

but as I have read in papers its in % not as above [i.e. 4.375 x10^4]

Similarly for PDT

the formula is
TD= t x log 2/log (Nt/N0)
where,
Nt= end point cell mo:
N0= initial cell number
t= time interval

now the Nt= 7468
N0= 2000

48 x 0.3010/ log (7468/2000)
48 x 0.3010/ log 3.734
0.572/ log 3.734
= 25.25

what does the following value indicate, Please help me out

its urgent


If I am wrong plz correct me
"Don't tell me sky is the limit when there is footprints on the moon"





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