Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Isolating Genomic DNA from a small colony of cultured human cells

  • Please log in to reply
1 reply to this topic

#1 stguest



  • Members
  • Pip
  • 3 posts

Posted 27 September 2011 - 11:25 AM

I have some small colonies of breast cancer cells growing in culture that I want to isolate genomic DNA from. I plan to use the isolated genomic DNA as template for PCR reactions. I'm assuming that the genomic DNA will need to first be amplified before it can be used as template considering my colonies consist of ~100cells. Can anyone point me to protocols or kits that will allow me isolate and amplify genomic DNA from this small number of cultured human cells?

Edited by stguest, 27 September 2011 - 11:26 AM.

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,709 posts

Posted 27 September 2011 - 02:51 PM

You shouldn't need to amplify the DNA, 100 cells =100 genomes, which should be enough DNA for a few PCRs.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.