Posted 27 September 2011 - 06:10 AM
Is it normal that, performing a phage display library and after X-gal selection, in the amplification reaction appears more fragments without the insert (around 240 bp) than with the insert (around 290 bp)?? in which step could the insert be broken away?
Posted 27 September 2011 - 10:07 PM
What library are you using - NEB PhD?
Posted 28 September 2011 - 02:08 AM
Posted 28 September 2011 - 02:47 AM
Posted 28 September 2011 - 03:11 AM
How is it posible that in a library like this appear to be such amount of insertless phages?? maybe a stupid question..
Posted 28 September 2011 - 03:17 AM
There is a very good paper on this subject that I recommend to anyone = Levitan - Stochastic modeling and optimization of phage display. Otherwise, I did a big part of my PhD with phage display, however the successes were few and far between.
Also tagged with one or more of these keywords: phage display, colony screening
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