PCR primer with no extra DNA end
Posted 27 September 2011 - 05:21 AM
Posted 27 September 2011 - 06:16 AM
XbaI does cut at much lower levels with 0 or 1 bases by the site, but it may still work. Alternatively, you could clone the PCR product into a T-Tailed vector like pGEM-T easy and then digest your fragment out of the plasmid.
Best of Luck.
Posted 27 September 2011 - 06:28 AM
Posted 02 October 2011 - 02:40 AM
I didnt work. So I think you are right. Fallback option now: assuming that the Xba1 site is still blunted.
Posted 02 October 2011 - 06:25 AM
You could consider TA cloning if you are using a Taq based pcr enzyme.